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References and Links to Papers

Reengineering of an Artificial Protein Cage for Efficient Packaging of Active Enzymes

Azuma Y, Gaweł S, Pasternak M, Woźnicka O, Pyza E, and Heddle JG*

Small 2024, 2312286 [doi: 10.1002/smll.202312286]

Complementary Charge-driven Encapsulation of Functional Protein by Engineered Protein Cages in Cellulo

Zakaszewski D, Koziej LPankowski J, Malolan VV, Gämperli N, Heddle JG, Hilvert D, and Azuma Y*
J. Mater. Chem. B, 2023, 11, 6540-6546, [doi: 10.1039/D3TB00754E]

Lumazine Synthase Protein Nanocompartments "Book chapter"
Koziej L, Gawin A, and Azuma Y*
In Microbiology Monographs, Microbial Production of High-Value Products 2022, 37, Wibowo D and Rehm BHA (Eds), Springer-Nature Switzerland AG, Cham, pp335-355 [doi: 10.1007/978-3-031-06600-9_13]

Chemically Induced Protein Cage Assembly with Programmable Opening and Cargo Release 
Stupka I, Azuma Y, Biela AP, Imanura M, Pyza E, Wożnicka O, Maskell DP, and Heddle JG*
Sci. Adv. 2022, 8, abj9424 [doi: 10.1126/sciadv.abj9424]   

Connectability of Protein Cages "Review article"
Majsterkiewicz K, Azuma Y and Heddle JG*
Nanoscale Adv., 2020, 2, 2255-2264[doi: 10.1039/D0NA00227E]

Cytoplasmic Glycoengineering Enables Biosynthesis of Nanoscale Glycoprotein Assemblies
Tytgat HLP, Lin C, Levasseur MD, Mock J, Terasaka N, Liebscher N, Azuma Y, Wetter M, Bachmann MF, Hilvert D, Aebi M, and Keys TG*
Nat. Commun. 2019, 10, 5403. [doi: 10.1038/s41467-019-13283]

Dipicolylamine/metal Complexes that Promote Direct Cell-membrane Penetration of Octaarginine
Kawaguchi Y, Ise S, Azuma Y, Takeuchi T, Kawano K, Le TK, Ohkanda J, and Futaki S*
Bioconjug. Chem. 2019, 30, 454-460. [doi: 10.1021/acs.bioconjchem.8b0069]

Modular Redesign of a Cationic Lytic Peptide to Promote Endosomal Escape of Biomacromolecules
Azuma Y, Imai H, Kawaguchi Y, Nakase I, Kimura H, and Futaki S*
Angew. Chem. Int. Ed. 2018, 57, 12771 –12774. [doi: 10.1002/anie.201807534]

Tailoring Lumazine Synthase Assemblies for Bionanotechnology “Review article”
Azuma Y, Edwardson TGW, and Hilvert D*
Chem. Soc. Rev. 2018, 47, 3543-3557. [doi: 10.1039/c8cs00154e]

Laboratory Evolution of Virus-like Nucleocapsids from Non-viral Protein Cages
Terasaka N, Azuma Y, and Hilvert D*
Proc. Natl. Acad. Sci. U. S. A. 2018, 115, 5432-5437. [doi: 10.1073/pnas.180052711]

Substrate Sorting by a Supercharged Nanoreactor
Azuma Y, Bader DLV, and Hilvert D*
J. Am. Chem. Soc. (ACS editor’s choice) 2018, 140, 860–863. [doi: 10.1021/jacs.7b11210]
[Featured in Nat. Catalysis 2018, 1, 94, and J. Am. Chem. Soc. 2018, 140, 1567]

Diversification of Protein Cage Structure Using Circularly Permuted Subunits
Azuma Y, Herger M, and Hilvert D*
J. Am. Chem. Soc. 2018, 140, 558–561. [doi: 10.1021/jacs.7b1051]

Modular Protein Cages for Size-Selective RNA Packaging in Vivo
Azuma Y, Edwardson TGW, Terasaka N, and Hilvert D*
J. Am. Chem. Soc. 2018, 140, 566–569. [doi: 10.1021/jacs.7b1079]

Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage “Book chapter”
Azuma Y, and Hilvert D*
In Methods Mol. Biol. 2018, 1798, Protein Scaffolds, A. K. Udit (Eds), Humana Press, New York, pp 39-55. [doi: 10.1007/978-1-4939-7893-9_4]
The C-terminal Peptide of Aquifex aeolicus Riboflavin Synthase Directs Encapsulation of Native and Foreign Guests by a Cage-forming Lumazine Synthase
Azuma Y, Zschoche R, and Hilvert D*
J. Biol. Chem. 2017, 292, 10321–10327. [doi: 10.1074/jbc.C117.79031]
Quantitative Packaging of Active Enzymes in a Protein Cage
Azuma Y, Zschoche R, Tinzl M, and Hilvert D*
Angew. Chem. Int. Ed. (Hot Paper) 2016, 55, 1531-1534. [doi: 10.1002/anie.20150841]
Controlling Leucine-zipper Partner Recognition in Cells through Modifications of a-g Interactions
Azuma Y, Kükenshöner T, Ma G, Yasunaga J, Imanishi M, Tanaka G, Nakase I, Maruno T, Kobayashi Y, Arndt KM*, Matsuoka M*, and Futaki S*
Chem. Commun. 2014, 50, 6364-6367. [doi: 10.1039/C4CC00555D]
Dipicolylamine as a Unique Structural Element for Helical Peptides
Azuma Y, Imai H, Yoshimura T, Kawabata T, Imanishi M, and Futaki S*
Org. Biomol. Chem. 2012, 10, 6062-6068. [doi: 10.1039/C2OB07118E]
Enhanced Target-Specific Accumulation of Radio Labeled Antibodies by Conjugating Arginine-Rich Peptides as Anchoring Molecules
Miyamoto R, Akizawa H, Nishikawa T, Uehara T, Azuma Y, Nakase I, Futaki S, Hanaoka H, Iida Y, Endo K, and Arano Y*
Bioconjug. Chem. 2010, 21, 2031-2037. [doi: 10.1021/bc100259q]
Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid
Azuma Y, Imanishi M, Yoshimura T, Kawabata T, and Futaki S*
Angew. Chem. Int. Ed. 2009, 48, 6853-6856. [doi: 10.1002/anie.20090288]
Hydrophilic Diazirine Polymer for One-Step Photo-Fabrication of Proteins on Polypropylene Surface
Tomohiro T, Tachi N, Azuma Y, and Hatanaka Y*
Heterocycles 2009, 79, 897-908. [doi: 10.3987/COM-08-S(D)60]


24 August, 2023, the Hague, the Netherlands

  1. Yusuke Azuma, "Triggerable morphology change of engineered lumazine synthase compartments"

31 March, 2023, Krakow, Poland

  1. Yusuke Azuma "Modular redesign of a cage-forming lumazine synthase for bionanotechnology"


22-28 January, 2023, Barga, Italy

  1. Agnieszka Gawin-Grebla and Yusuke Azuma "Encapsidic production of prone-to-degradation proteins", poster presentation

  2. Lukasz Koziej, Farzad Fatehi, Marta Aleksejczuk, Reidun Twarock, and Yusuke Azuma "Polymorphology of capsid-like assemblies formed by an engineered lumazine synthase", poster presentation


17-18 August, 2022, Cancun, Mexico (hybrid)

  1. Yusuke Azuma, and Jonathan Heddle "Artificial protein cages with programmed disassembling characteristics", invited talk

  2. Marta Aleksejczuk, Lukasz Koziej, and Yusuke Azuma, "Controlled assembly of an engineered lumazine synthase", poster and video presentation

  3. Jędrzej Pankowski, Agnieszka Gawin, and Yusuke Azuma, "A whole cell-based method to evaluate the function of guest proteins encapsulated in engineered proteinaceous containers., poster and video presentation

  4. Vishal Malolan V, and Yusuke Azuma, "Re-engineering of lumazine synthase protein cages towards triggered disassembly", poster and video presentation

  5. Agnieszka Gawin, and Yusuke Azuma, "Circularly permuted lumazine synthase as a tool for construction of artificial subcellular compartments with new capability", poster and video presentation

  6. Daniel Zakaszewski, Lukasz Koziej, and Yusuke Azuma, "Complementary charge-driven guest encapsulation by protein cages for construction of artificial subcellular compartments", poster and video presentation   


21-22 October, 2021, Warsaw, Poland

  1. Marta Aleksejczuk, Lukasz Koziej, Yusuke Azuma "Dynamics of engineered lumazine synthase assemblies", poster presentation

  2. Yusuke Azuma "Remodeling, Assembly, and CryoEM Structure of a Cage-forming Lumazine Synthase", Short talk


8 October, 2020, online

  1. Yusuke Azuma "Guest Packaging and Structure of Engineered Bacterial Nanocompartments" oral presentation


02-04 September 2020, online

  1. Yusuke Azuma "Guest packaging mechanism of the bacterial compartment for vitamin B2 biosynthesis", oral presentation

  2. Łukasz Koziej "Cryo-EM study on packaging and assembly of cage-forming lumazine synthase", flash talk


International Patent

An artificial Protein-cage comprising encapsulated therein a guest cargo

Heddle J, Biela A, Azuma Y, Naskalska A, Borzęcka-Solarz K, Różycki J, Stupka I

Application, PCT/PL2022/050011, 24.02.2022

Publication, WO 2022/182262 A1, 01.09.2022

International Patent 

An artificial TRAP-cage, its use and method of preparing thereof

Heddle J, Biela A, Azuma Y, Stupka I, Majsterkiewicz

Application, PCT/PL2022/050010, 24.02.2022

Publication, WO 2022/182261 A1, 01.09.2022

International Patent

An artificial Protein-cage decorated with particular molecules on the exterior

Heddle J, Azuma Y, Naskalska A, Borzęcka-Solarz K, Różycki J, Gaweł S

Application, PCT/PL2022/050009, 24.02.2022

Publication, WO 2022/182260 A1, 01.09.2022

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