PUBLICATIONS

Encapsidic Production and Isolation of Degradation-prone Polypeptides

Gawin A, Pankowski J, Zarechyntsava M, Kwasna D, Kloska D, Koziej L, Glatt S, Kachamakova-Trojanowska N, and Azuma Y*

BioRxiv [doi: 10.1101/2025.02.04.636450]

​​

Dynamic Assembly of Pentamer-based Protein Nanotubes

Koziej L, Fatehi F, Aleksejczuk M, Byrne MJ, Heddle JG, Twarock R, and Azuma Y*

ACS Nano 2025, 19, 8786–8798 [doi: 10.1021/acsnano.4c16192] 

An earlier version of the manuscript was deposited to BioRxiv [doi: 10.1101/2024.08.15.608090]

​

Designed, Programmable Protein Cages Utilizing Diverse Metal Coordination Geometries Show Reversible, pH-Dependent Assembly

Osiński N, Majsterkiewicz K, Pakosz-Stępień Z, Azuma Y, Biela AP, Gaweł S, and Heddle JG*

Macromol. Rapid Commun. 2024, 2400712 [doi: 10.1002/marc.202400712]

​​​

Reengineering of an Artificial Protein Cage for Efficient Packaging of Active Enzymes

Azuma Y, Gaweł S, Pasternak M, Woźnicka O, Pyza E, and Heddle JG*

Small 2024, 2312286 [doi: 10.1002/smll.202312286]

​

Complementary Charge-driven Encapsulation of Functional Protein by Engineered Protein Cages in Cellulo

Zakaszewski D, Koziej L, Pankowski J, Malolan VV, Gämperli N, Heddle JG, Hilvert D, and Azuma Y*
J. Mater. Chem. B, 2023, 11, 6540-6546, [doi: 10.1039/D3TB00754E]

Lumazine Synthase Protein Nanocompartments "Book chapter"
Koziej L, Gawin A, and Azuma Y*
In Microbiology Monographs, Microbial Production of High-Value Products 2022, 37, Wibowo D and Rehm BHA (Eds), Springer-Nature Switzerland AG, Cham, pp335-355 [doi: 10.1007/978-3-031-06600-9_13]

Chemically Induced Protein Cage Assembly with Programmable Opening and Cargo Release 
Stupka I, Azuma Y, Biela AP, Imanura M, Pyza E, Wożnicka O, Maskell DP, and Heddle JG*
Sci. Adv. 2022, 8, abj9424 [doi: 10.1126/sciadv.abj9424]   

Connectability of Protein Cages "Review article"
Majsterkiewicz K, Azuma Y and Heddle JG*
Nanoscale Adv., 2020, 2, 2255-2264. [doi: 10.1039/D0NA00227E]

Cytoplasmic Glycoengineering Enables Biosynthesis of Nanoscale Glycoprotein Assemblies
Tytgat HLP, Lin C, Levasseur MD, Mock J, Terasaka N, Liebscher N, Azuma Y, Wetter M, Bachmann MF, Hilvert D, Aebi M, and Keys TG*
Nat. Commun. 2019, 10, 5403. [doi: 10.1038/s41467-019-13283]

Dipicolylamine/metal Complexes that Promote Direct Cell-membrane Penetration of Octaarginine
Kawaguchi Y, Ise S, Azuma Y, Takeuchi T, Kawano K, Le TK, Ohkanda J, and Futaki S*
Bioconjug. Chem. 2019, 30, 454-460. [doi: 10.1021/acs.bioconjchem.8b0069]

Modular Redesign of a Cationic Lytic Peptide to Promote Endosomal Escape of Biomacromolecules
Azuma Y, Imai H, Kawaguchi Y, Nakase I, Kimura H, and Futaki S*
Angew. Chem. Int. Ed. 2018, 57, 12771 –12774. [doi: 10.1002/anie.201807534]

Tailoring Lumazine Synthase Assemblies for Bionanotechnology “Review article”
Azuma Y, Edwardson TGW, and Hilvert D*
Chem. Soc. Rev. 2018, 47, 3543-3557. [doi: 10.1039/c8cs00154e]

Laboratory Evolution of Virus-like Nucleocapsids from Non-viral Protein Cages
Terasaka N, Azuma Y, and Hilvert D*
Proc. Natl. Acad. Sci. U. S. A. 2018, 115, 5432-5437. [doi: 10.1073/pnas.180052711]

Substrate Sorting by a Supercharged Nanoreactor
Azuma Y, Bader DLV, and Hilvert D*
J. Am. Chem. Soc. (ACS editor’s choice) 2018, 140, 860–863. [doi: 10.1021/jacs.7b11210]
[Featured in Nat. Catalysis 2018, 1, 94, and J. Am. Chem. Soc. 2018, 140, 1567]

Diversification of Protein Cage Structure Using Circularly Permuted Subunits
Azuma Y, Herger M, and Hilvert D*
J. Am. Chem. Soc. 2018, 140, 558–561. [doi: 10.1021/jacs.7b1051]

Modular Protein Cages for Size-Selective RNA Packaging in Vivo
Azuma Y, Edwardson TGW, Terasaka N, and Hilvert D*
J. Am. Chem. Soc. 2018, 140, 566–569. [doi: 10.1021/jacs.7b1079]

Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage “Book chapter”
Azuma Y, and Hilvert D*
In Methods Mol. Biol. 2018, 1798, Protein Scaffolds, A. K. Udit (Eds), Humana Press, New York, pp 39-55. [doi: 10.1007/978-1-4939-7893-9_4]
 
The C-terminal Peptide of Aquifex aeolicus Riboflavin Synthase Directs Encapsulation of Native and Foreign Guests by a Cage-forming Lumazine Synthase
Azuma Y, Zschoche R, and Hilvert D*
J. Biol. Chem. 2017, 292, 10321–10327. [doi: 10.1074/jbc.C117.79031]
 
Quantitative Packaging of Active Enzymes in a Protein Cage
Azuma Y, Zschoche R, Tinzl M, and Hilvert D*
Angew. Chem. Int. Ed. (Hot Paper) 2016, 55, 1531-1534. [doi: 10.1002/anie.20150841]
 
Controlling Leucine-zipper Partner Recognition in Cells through Modifications of a-g Interactions
Azuma Y, Kükenshöner T, Ma G, Yasunaga J, Imanishi M, Tanaka G, Nakase I, Maruno T, Kobayashi Y, Arndt KM*, Matsuoka M*, and Futaki S*
Chem. Commun. 2014, 50, 6364-6367. [doi: 10.1039/C4CC00555D]
 
Dipicolylamine as a Unique Structural Element for Helical Peptides
Azuma Y, Imai H, Yoshimura T, Kawabata T, Imanishi M, and Futaki S*
Org. Biomol. Chem. 2012, 10, 6062-6068. [doi: 10.1039/C2OB07118E]
 
Enhanced Target-Specific Accumulation of Radio Labeled Antibodies by Conjugating Arginine-Rich Peptides as Anchoring Molecules
Miyamoto R, Akizawa H, Nishikawa T, Uehara T, Azuma Y, Nakase I, Futaki S, Hanaoka H, Iida Y, Endo K, and Arano Y*
Bioconjug. Chem. 2010, 21, 2031-2037. [doi: 10.1021/bc100259q]
 
Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid
Azuma Y, Imanishi M, Yoshimura T, Kawabata T, and Futaki S*
Angew. Chem. Int. Ed. 2009, 48, 6853-6856. [doi: 10.1002/anie.20090288]
 
Hydrophilic Diazirine Polymer for One-Step Photo-Fabrication of Proteins on Polypropylene Surface
Tomohiro T, Tachi N, Azuma Y, and Hatanaka Y*
Heterocycles 2009, 79, 897-908. [doi: 10.3987/COM-08-S(D)60]