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References and Links to Papers

Lumazine Synthase Protein Nanocompartments "Book chapter"

Koziej L, Gawin A, and Azuma Y*

In Microbiology Monographs, Microbial Production of High-Value Products 2022, 37, Wibowo D and Rehm BHA (Eds), Springer-Nature Switzerland AG, Cham, pp335-355 []

Chemically Induced Protein Cage Assembly with Programmable Opening and Cargo Release 

Stupka I, Azuma Y, Biela AP, Imanura M, Pyza E, Wożnicka O, Maskell DP, and Heddle JG*

Sci. Adv. 2022, 8, abj9424 [doi: 10.1126/sciadv.abj9424]   

Connectability of Protein Cages "Review article"

Majsterkiewicz K, Azuma Y and Heddle JG*

Nanoscale Adv., 2020, 2, 2255-2264[doi: 10.1039/D0NA00227E]

Cytoplasmic Glycoengineering Enables Biosynthesis of Nanoscale Glycoprotein Assemblies

Tytgat HLP, Lin C, Levasseur MD, Mock J, Terasaka N, Liebscher N, Azuma Y, Wetter M, Bachmann MF, Hilvert D, Aebi M, and Keys TG*

Nat. Commun. 2019, 10, 5403. [doi: 10.1038/s41467-019-13283]

Dipicolylamine/metal Complexes that Promotes Direct Cell-membrane Penetration of Octaarginine

Kawaguchi Y, Ise S, Azuma Y, Takeuchi T, Kawano K, Le TK, Ohkanda J, and Futaki S*

Bioconjug. Chem. 2019, 30, 454-460. [doi: 10.1021/acs.bioconjchem.8b0069]

Modular Redesign of a Cationic Lytic Peptide to Promote Endosomal Escape of Biomacromolecules

Azuma Y, Imai H, Kawaguchi Y, Nakase I, Kimura H, and Futaki S*

Angew. Chem. Int. Ed. 2018, 57,12771 –12774. [doi: 10.1002/anie.201807534]

Tailoring Lumazine Synthase Assemblies for Bionanotechnology “Review article”

Azuma Y, Edwardson TGW, and Hilvert D*

Chem. Soc. Rev. 2018, 47, 3543-3557. [doi: 10.1039/c8cs00154e]

Laboratory Evolution of Virus-like Nucleocapsids from Non-viral Protein Cages

Terasaka N, Azuma Y, and Hilvert D*

Proc. Natl. Acad. Sci. U. S. A. 2018, 115, 5432-5437. [doi: 10.1073/pnas.180052711]

Substrate Sorting by a Supercharged Nanoreactor

Azuma Y, Bader DLV, and Hilvert D*

J. Am. Chem. Soc. (ACS editor’s choice) 2018, 140, 860–863. [doi: 10.1021/jacs.7b11210]

[Featured in Nat. Catalysis 2018, 1, 94, and J. Am. Chem. Soc. 2018, 140, 1567]

Diversification of Protein Cage Structure Using Circularly Permuted Subunits

Azuma Y, Herger M, and Hilvert D*

J. Am. Chem. Soc. 2018, 140, 558–561. [doi: org/10.1021/jacs.7b1051]

Modular Protein Cages for Size-Selective RNA Packaging in Vivo

Azuma Y, Edwardson TGW, Terasaka N, and Hilvert D*

J. Am. Chem. Soc. 2018, 140, 566–569. [doi: 10.1021/jacs.7b1079]

Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage “Book chapter”

Azuma Y, and Hilvert D*

In Methods Mol. Biol. 2018, 1798, Protein Scaffolds, A. K. Udit (Eds), Humana Press, New York, pp 39-55. [doi: 10.1007/978-1-4939-7893-9_4]

The C-terminal Peptide of Aquifex aeolicus Riboflavin Synthase Directs Encapsulation of Native and Foreign Guests by a Cage-forming Lumazine Synthase

Azuma Y, Zschoche R, and Hilvert D*

J. Biol. Chem. 2017, 292, 10321–10327. [doi: 10.1074/jbc.C117.79031]

Quantitative Packaging of Active Enzymes in a Protein Cage

Azuma Y, Zschoche R, Tinzl M, and Hilvert D*

Angew. Chem. Int. Ed. (Hot Paper) 2016, 55, 1531-1534. [doi: 10.1002/anie.20150841]

Controlling Leucine-zipper Partner Recognition in Cells through Modifications of a-g Interactions

Azuma Y, Kükenshöner T, Ma G, Yasunaga J, Imanishi M, Tanaka G, Nakase I, Maruno T, Kobayashi Y, Arndt KM*, Matsuoka M*, and Futaki S*

Chem. Commun. 2014, 50, 6364-6367. [doi: 10.1039/C4CC00555D]

Dipicolylamine as a Unique Structural Element for Helical Peptides

Azuma Y, Imai H, Yoshimura T, Kawabata T, Imanishi M, and Futaki S*

Org. Biomol. Chem. 2012, 10, 6062-6068. [doi: 10.1039/C2OB07118E]

Enhanced Target-Specific Accumulation of Radio Labeled Antibodies by Conjugating Arginine-Rich Peptides as Anchoring Molecules

Miyamoto R, Akizawa H, Nishikawa T, Uehara T, Azuma Y, Nakase I, Futaki S, Hanaoka H, Iida Y, Endo K, and Arano Y*

Bioconjug. Chem. 2010, 21, 2031-2037. [doi: 10.1021/bc100259q]

Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid

Azuma Y, Imanishi M, Yoshimura T, Kawabata T, and Futaki S*

Angew. Chem. Int. Ed. 2009, 48, 6853-6856. [doi: 10.1002/anie.20090288]

Hydrophilic Diazirine Polymer for One-Step Photo-Fabrication of Proteins on Polypropylene Surface

Tomohiro T, Tachi N, Azuma Y, and Hatanaka Y*

Heterocycles 2009, 79, 897-908. [doi: 10.3987/COM-08-S(D)60]

Publications: Publications


31 March, 2023, Krakow, Poland

  1. Yusuke Azuma "Modular redesign of a cage-forming lumazine synthase for bionanotechnology"

22-28 January, 2023, Barga, Italy

  1. Agnieszka Gawin-Grebla and Yusuke Azuma "Encapsidic production of prone-to-degradation proteins", poster presentation

  2. Lukasz Koziej, Farzad Fatehi, Marta Aleksejczuk, Reidun Twarock, and Yusuke Azuma "Polymorphology of capsid-like assemblies formed by an engineered lumazine synthase", poster presentation

17-18 August, 2022, Cancun, Mexico (hybrid)

  1. Yusuke Azuma, and Jonathan Heddle "Artificial Protein Cages with Programmed Disassembling Characteristics", invited talk

  2. Marta Aleksejczuk, Lukasz Koziej, and Yusuke Azuma, "Controlled assembly of an engineered lumazine synthase", poster and video presentation

  3. Jędrzej Pankowski, Agnieszka Gawin, and Yusuke Azuma, "A whole cell-based method to evaluate the function of guest proteins encapsulated in engineered proteinaceous containers., poster and video presentation

  4. Vishal Malolan V, and Yusuke Azuma, "Re-engineering of lumazine synthae protein cages towards triggered disassembly", poster and video presentation

  5. Agnieszka Gawin, and Yusuke Azuma, "Circularly permuted lumazine synthase as a tool for construction of artificial subcellular compartments with new capability", poster and video presentation

  6. Daniel Zakaszewski, Lukasz Koziej, and Yusuke Azuma, "Complementary charge-driven guest encapsulation by protein cages for construction of artificial subcellular compartments", poster and video presentation   

21-22 October, 2021, Warsaw, Poland

  1. Marta Aleksejczuk, Lukasz Koziej, Yusuke Azuma "Dynamics of engineered lumazine synthase assemblies", poster presentation

  2. Yusuke Azuma "Remodeling, Assembly, and CryoEM Structure of a Cage-forming Lumazine Synthase", Short talk

8 October, 2020, online

Yusuke Azuma "Guest Packaging and Structure of Engineered Bacterial Nanocompartments" oral presentation

02-04 September 2020, online

  1. Yusuke Azuma "Guest packaging mechanism of the bacterial compartment for vitamin B2 biosynthesis", oral presentation

  2. Łukasz Koziej "Cryo-EM study on packaging and assembly of cage-forming lumazine synthase", flash talk

Publications: Publications


International Patent

An artificial Protein-cage comprising encapsulated therein a guest cargo

Heddle J, Biela A, Azuma Y, Naskalska A, Borzęcka-Solarz K, Różycki J, Stupka I

Application, PCT/PL2022/050011, 24.02.2022

Publication, WO 2022/182262 A1, 01.09.2022

International Patent 

An artificial TRAP-cage, its use and method of preparing thereof

Heddle J, Biela A, Azuma Y, Stupka I, Majsterkiewicz

Application, PCT/PL2022/050010, 24.02.2022

Publication, WO 2022/182261 A1, 01.09.2022

International Patent

An artificial Protein-cage decorated with particular molecules on the exterior

Heddle J, Azuma Y, Naskalska A, Borzęcka-Solarz K, Różycki J, Gaweł S

Application, PCT/PL2022/050009, 24.02.2022

Publication, WO 2022/182260 A1, 01.09.2022

Publications: Publications
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